TAGS & MODIFIED BASES

The stepwise coupling efficiencies of the minor nucleotides are generally less than the efficiencies we guarantee for normal DNA bases (G,A,T,C). As a consequence, the yield of full length oligo may be reduced relative to an oligo that does not contain a minor nucleotide. While we lot-select components to obtain the highest possible coupling efficiencies and yields of these minor nucleotides, the gel photos may show less than full length bands corresponding to the positions of the minor nucleotides in the sequence.

All oligos are synthesized from the 3' end on solid supports. Selecting a modified 3' end is not recommended, particularly at the jumbo synthesis scale, as this may reduce quality and yield.

If there is a modifier that you would like, but it is not in the following list, please ask us. If a 3' modification is essential to your project, please contact our technical staff. Inquire for pricing.

5' and internal AMINO MODIFIERS

• Amino dT C2 Linker
• Amino dT C6 Linker
• Amino dC C6 Linker
• Amino C-3 Linker
• C-5 Amino Modifier
• Amino C-6 Linker
• Amino C-6 TFA Linker
• Amino C-12 Linker

3' AMINO MODIFIERS

• 3' Amino cT C6 Linker
• 3' Amino dC C6 Linker
• 3' C3 Amino Modifier
• 3' C7 Amino Modifier

BIOTIN MODIFIERS

• 5' Biotin
• Biotin Amidite
• Biotin-ON
• Biotin-TEG
• 3' Biotin TEG
• Biotin dT

CONJUGATES

• BODIPY's
• Cholesterol
• Digoxigenin (Licensed from Boehringer Mannheim)
• OREGON GREEN (Licensed from Molecular Probes)
• 5' Rhodamine (available as post-synthetic labels)
• Rhodamine dU (available as post-synthetic labels)
• 3' Rhodamine (available as post-synthetic labels)
• Texas Red
• TAMRA

FLUORESCENT TAGS - These dyes can be added during synthesis

• 5' Acridine
• 3' Acridine
• 3' Dabcyl
• 5' 6-FAM
• 5' Fluorescein
• 3' Fluorescein
• 5' HEX
• Psoralen 5'
• Psoralen 5' C-6
• 5' TET
• 3'6 FAM
• 3' Fluorescein dT
• 3'6 Fluorescein
• 5'6 Fluorescein
• 5' Fluorescein Mixed Isomer

• 3' Dabsyl
• 3' Dabcyl
• Dabcyl dT
• 5' Dabcyl

MINOR "NUCLEOTIDES"

• 5' Amino-dT
• 5-Bromo-deoxy Cytidine
• 5-Bromo-deoxy Uridine
• 8-Bromo-dA
• 8-Bromo-dG
• Carboxy dT
• 5' dA
• 5' dC
• 5' dG
• 5' dT
• 7-deaza-deoxy Adenosine
• 7-deaza-deoxy Guanosine
• Degenerate positions
• 2' deoxy-Inosine
• 5' Digoxigenin (Licensed from Boehringer Mannheim)
• Etheno dA
• 5-Fluoro-deoxy Uridine
• 5 Hydroxy dC
• 5 Hydroxy dU
• 5' Hydroxymethyl-dU
• 5-Iodo-deoxy Cytidine
• 5-Iodo-deoxy Uridine
• 5-Methyl-deoxy Cytidine
• 5-Methyl-iso dC
• O-6 Methyl-deoxy Guanosine*
• O-4 Methyl dT
• 5'-O-Methyl dT
• N-6-Methyl-2'-deoxy Adensine
• 5 Nitropyrrole
• 3 Nitroindole
• 5' Phosphate
• 2' deoxy-Nebularine
• Ribo-Inosine
• S6-DNP dG
• 2' deoxy-Uridine

*Requires customer deprotection

3' MODIFIERS

• C-11 CPG
• 3' Cholesterol
• 3' Cordecypin CPGs
• dI CPG
• dU CPG
• 3' Phosphate
• 3' RNA CPG

THIOL MODIFIERS

• 4 Thio dU
• 4 Thio dT
• 6 Thio dG
• 5' Thiol C-6 Linker
• 5' Thiol C-6 S-S
• 3' Thiol C3 S-S

SPACERS

• dSpacer
• Spacer 9
• Spacer 18
• Spacer C12
• Symmetric Branch
• Trebler

SPECIAL BACKBONE MODIFICATIONS

• chimeric oligonucleotides
• 5'-5' linkages
• 3'-3' linkages
• 5'-2' linkages
• 2'-O-methy RNA
• p-ethoxy DNA
• phosphorothioate
• RNA
• Second Generation Optimized Oligonucleotides

Please inquire for modifiers, fluorescent dyes, or quenchers not on this list.

Please inquire for RNA or 2' Omethyl RNA modifiers not on this list.