PURIFICATION

Primer Grade (NO CHARGE)

Organic extraction, precipitation, and two vacuum desiccation steps are used to remove small molecule contaminants from every oligonucleotide.

GEL PURIFICATION (Research Grade)

Gel purification is the most economical way to purify oligonucleotides for research needs. It is currently the best purification choice for longer oligonucleotides (>40 bases) and for oligonucleotides in which secondary structure may substantially interfere with HPLC purification.

Standard HPLC Purification Grades*

80% - 90%
Level I 90 - <95% Pure

Level II 95 - 99+% Pure

**Gel photograph and analytical HPLC traces are sent.

Capillary electrophoresis traces are provided.

There are yield differences with each HPLC purification level. When increasing the percent purity (i.e., from level I to level II), the yield decreases. Longer lead times may be required for higher purity levels, especially if process development is undertaken. Final yields can be sequence dependent.

*Other minimum purity levels are available with prior approval from our Technical Services Department.